Comments on: Saturday Nov 12, #SFN11, poster A27 = me

By: Dina Wingfield

Dina Wingfield — Mon, 05 Dec 2011 15:12:43 +0000

My daughter will be graduating with a neuroscience degree and is hoping for a career in neuroradiology. I am wondering if you would consider selling any of your images as prints for framing??? (looking for Christmas or graduation gifts) Let me know. Thanks.

By: Neuroskeptic

Neuroskeptic — Sun, 13 Nov 2011 10:15:58 +0000

That top one is very pretty.

By: Patrick

Patrick — Wed, 09 Nov 2011 22:22:17 +0000

That first paper is great. We’re seeing something similar, in that we have a mixed population, so that is encouraging. Not sure how Tubb3 and Hu expression related, but that’s something we can figure out…

thanks!
patrick

By: Jason Snyder

Jason Snyder — Wed, 09 Nov 2011 13:49:12 +0000

I haven’t done that myself but I did see recent paper by Seki that describes something similar, using GFAP and the neuronal marker Hu: http://www.ncbi.nlm.nih.gov/pubmed/21966492 and http://www.ncbi.nlm.nih.gov/pubmed/20026190

By: patrick

patrick — Wed, 09 Nov 2011 11:20:31 +0000

Jason, as usual spectacular images. We are producing enriched vGluT2+ neurons from suspension cultured ES cells for neurotox type of studies. Immediately after differentiation and plating we see a radial glial-like morphology, followed by development of traditional pyramidal neuron-like characteristics. In the first few days we see what *appears* to be co-expression of GFAP and b3 tubulin in many of these and are now trying to identify if the GFAP signal is real, or an artifact. Have you co-stained radial glia in situ for putative astrocyte and early neuronal markers? If so, what have you observed?

By: Leonora

Leonora — Wed, 09 Nov 2011 07:06:37 +0000

Very pretty pictures – I like the white stain particularly :)

So I’m interested in these radial glia as neuronal precursors – I didn’t know that dentate gyrus neurons were glia beforehand. I’d love to learn more! Is there a percentage of these glia that don’t differentiate into neurons? Does this differ in the SVZ vs the dentate gyrus? And is there some functional role that these glia carry out before they differentiate into neurons, like guiding migration (as you see in the cortex)?