Olfactory bulb neurogenesis big bigger biggest

And now for a journey outside (rostral, to be precise) of my comfort zone. These three pictures show new neurons in the mouse olfactory bulb at successively greater magnifications. Probably inspired by the science magazine I read as a kid that would show high mag photos of everyday objects (with corresponding low mag photos as the answers).

With a 10x objective I could capture nearly the entire bulb (saggital section) in a single field. You can see newborn BrdU+ cells (green) scattered throughout, most co-labeled with doublecortin (red). In the bottom left area you can see about a dozen glomeruli – groups of neurons that represent different odors, located just one synapse upstream of the nasal epithelium. Whereas the majority of adult-born olfactory neurons are inhibitory interneurons, a smaller number of new neurons surrounding the glomeruli (periglomerular neurons) are dopaminergic. (click on the images for full sized versions – 2048 x 2048 pixels)

low magnification doublecortin and BrdU

Zooming in with a 20x objective:

zoomed in

And then with a 60x objective. I apologize this photo could have been better but I can’t spend all day on this stuff.

BrdU-DCX colabeling high magnification

If I had a confocal microscope at home I’d never stop.

2 thoughts on “Olfactory bulb neurogenesis big bigger biggest”

  1. I have discovered how to manifest neurogenesis in the human brain with Meditation, Music Therapy and Aromatherapy with Certified Pure Therapeutic Grade essential oils. What can be done with these things is similar to Cranial Electrotherapy Stimulation, except without electricity. The electricity that is created in the brain is caused by moving the eyes when meditating to music, simultaneously inhaling the oils. Id be interested in discussing this with you.

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